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Table 1 qRT-PCR validation of microarray data

From: Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

Gene Symbol Microarray fold change qRT-PCR fold change Correlation*
  1 hr 3 hr 6 hr 24 hrs 1 hr 3 hr 6 hr 24 hrs  
IL1B 4.9 14.6 5.7 1.7 41.0 127.4 52.6 5.2 0.99
IL6 103.3 771.7 193.0 15.2 6.4 11.9 6.1 1.4 0.92
IL8 44.0 311.9 231.6 454.6 21.7 244.5 293.8 477.4 0.96
TNF 3.4 1.3 1.5 1.3 5.2 2 2.5 0.8 0.95
IL10 2.2 2.1 2.8 2.3 1.8 1.7 1.9 0.7 0.4
IL18 1.1 1.1 1.4 1.3 1.4 1.3 1.6 0.7 0.68
TGFBI 1.5 1.3 1.5 3.2 1.6 1.7 1.7 3.2 0.99
TLR4 1.3 2.3 2.0 1.2 0.8 1.5 1.2 0.5 0.96
TLR2 2.0 4.6 3.4 2.8 1.5 2.7 2.3 1.0 0.83
IL4 4.2 19.5 28.8 2.2 1.9 8.7 9.2 0.8 0.97
CSF2 6.7 4.9 4.4 1.6 9.2 9.1 9.8 1.4 0.86
  Mean correlation 0.87
  1. Key - Comparisons between baseline samples (time = 0) and individual time points. *Correlations performed using Pearson's correlation co-efficient for highly expressed genes, i.e. IL1B, IL6, IL8, TNF, TLR4, TLR2, IL4 and CSF2 and Spearman's rank correlation co-efficient for genes with lower expression levels, i.e. IL10, IL18 and TGFBI
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