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Table 1 qRT-PCR validation of microarray data

From: Transcriptomic analysis of the temporal host response to skin infestation with the ectoparasitic mite Psoroptes ovis

Gene Symbol

Microarray fold change

qRT-PCR fold change

Correlation*

 

1 hr

3 hr

6 hr

24 hrs

1 hr

3 hr

6 hr

24 hrs

 

IL1B

4.9

14.6

5.7

1.7

41.0

127.4

52.6

5.2

0.99

IL6

103.3

771.7

193.0

15.2

6.4

11.9

6.1

1.4

0.92

IL8

44.0

311.9

231.6

454.6

21.7

244.5

293.8

477.4

0.96

TNF

3.4

1.3

1.5

1.3

5.2

2

2.5

0.8

0.95

IL10

2.2

2.1

2.8

2.3

1.8

1.7

1.9

0.7

0.4

IL18

1.1

1.1

1.4

1.3

1.4

1.3

1.6

0.7

0.68

TGFBI

1.5

1.3

1.5

3.2

1.6

1.7

1.7

3.2

0.99

TLR4

1.3

2.3

2.0

1.2

0.8

1.5

1.2

0.5

0.96

TLR2

2.0

4.6

3.4

2.8

1.5

2.7

2.3

1.0

0.83

IL4

4.2

19.5

28.8

2.2

1.9

8.7

9.2

0.8

0.97

CSF2

6.7

4.9

4.4

1.6

9.2

9.1

9.8

1.4

0.86

 

Mean correlation

0.87

  1. Key - Comparisons between baseline samples (time = 0) and individual time points. *Correlations performed using Pearson's correlation co-efficient for highly expressed genes, i.e. IL1B, IL6, IL8, TNF, TLR4, TLR2, IL4 and CSF2 and Spearman's rank correlation co-efficient for genes with lower expression levels, i.e. IL10, IL18 and TGFBI