Experimental identification of F. tularensis sRNAs. (A) Total RNA extracted at exponential phase (E), stationary phase (S), high salt concentration (NaCl) or after exposure to oxidative stress (H2O2) analyzed by 8% PAGE and stained by ethidium bromide (M, RNA marker). Individual bands processed for cloning are indicated by numbers. (B) Schematic representation of gel used for cloning of cDNA. Each square denotes a piece that was individually processed for RNA extraction and A and B designate the area from which FtrA and B were identified, respectively. For clarification, the highly expressed RNAs (#1-3) seen in (A) are indicated.