Table 4 Bacterial strains and plasmids
From: Identification of small RNAs in Francisella tularensis
Strain or plasmid | Description | Source or reference | |
|---|---|---|---|
Strain | Â | Â | Â |
F. tularensis | Â | Â | |
|  | LVS | subsp. holarctica, live vaccine strain | A. Sjöstedt |
|  | LVSΔftrA | LVS with deletion of ftrA | This study |
|  | LVSΔftrB | LVS with deletion of ftrB | This study |
E. coli | Â | Â | |
|  | DH5α |  | Strain collection |
Plasmid | Â | Â | |
| Â | pCR2.1-TOPO | PCR cloning vector, KmR, AmpR | Invitrogen |
| Â | pMP812 | sacB suicide vector, KmR | [40] |
|  | pMP-ΔftrA | pMP812 containing ~2 kb fragment for deletion of ftrA | This study |
|  | pMP-ΔftrB | pMP812 containing ~2 kb fragment for deletion of ftrB | This study |