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Table 4 Bacterial strains and plasmids

From: Identification of small RNAs in Francisella tularensis

Strain or plasmid Description Source or reference
Strain    
F. tularensis   
  LVS subsp. holarctica, live vaccine strain A. Sjöstedt
  LVSΔftrA LVS with deletion of ftrA This study
  LVSΔftrB LVS with deletion of ftrB This study
E. coli   
  DH5α   Strain collection
Plasmid   
  pCR2.1-TOPO PCR cloning vector, KmR, AmpR Invitrogen
  pMP812 sacB suicide vector, KmR [40]
  pMP-ΔftrA pMP812 containing ~2 kb fragment for deletion of ftrA This study
  pMP-ΔftrB pMP812 containing ~2 kb fragment for deletion of ftrB This study