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Table 1 Templates used to interrogate shikimic acid pathway structure in the sequenced genomes of prokaryotes.

From: Global genome analysis of the shikimic acid pathway reveals greater gene loss in host-associated than in free-living bacteria

Shikimate Pathway Step Product Enzymes and Enzyme Isoforms Source and Genetic/Protein Templates
1 3-Deoxy-D-arabino-heptulosinate -7- phosphate (DAHP) DAHP synthase EC (aroF), (aroG), (aroH) In E. coli there are three DAHP synthetase isoforms, each specifically inhibited by one of the three aromatic amino acids.
   KDPGal aldolase EC See: and [12]
2 3-Dehydroquinate (DHQ) DHQ synthase EC (aroB) DHQ synthase exists as type 1 and 2 enzymes (previously EC
3 3-dehydroshikimate (DHS) Shikimate dehydrase EC (aroD) shikimate dehydrase and shikimate dehydrogenase are often a bifunctional enzyme
4 Shikimic acid (shikimate) Shikimate dehydrogenase EC (aroE) E. coli has the putative enzyme YdiB paralog [25]
5 Shikimate-3-phosphate Shikimate kinase II EC (aroL) monofunctional shikimate kinase
   Archaeal GHMP shikimate kinase See: and [11]
6 5-Enolpyruvyl-shikimate-3-phosphate (EPSP) EPSP Synthase EC (aroA) The AroA gene, coding for the E. coli EPSP synthase, was first isolated from a lambda transducing phage (lambda-serC) found to contain a portion of the E. coli chromosome
2-6 5-Enolpyruvyl-shikimate-3-phosphate (EPSP) Shikimate kinase I
EC (aroM)
Pentafunctional gene consisting of aroB, aroD, aroE, aroL and aroA
7 Chorismic acid (chorismate) Chorismate synthesis EC (aroC) previously EC Chorismate synthase from various sources shows a high degree of sequence conservation.