Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties

Biller, Laura; Davis, Paul H; Tillack, Manuela; Matthiesen, Jenny; Lotter, Hannelore; Stanley, Samuel L; Tannich, Egbert; Bruchhaus, Iris

doi:10.1186/1471-2164-11-63

Table 2 List of AIG1-like family members

From: Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties

	GenBank Accession no. Gene	GenBank Accession no. Protein	Size aa	G1	G2	G3	C1	C2	C3	Cc** (position aa)	TM*** (position aa)	Real-time PCR data (x-fold)
												Cell line A	Cell line B
A > B
1*	XM_643035	XP_648127	364	+	+	+	+	+	+			12.5
A < B
2	XM_648725	XP_653817	335	+	+	+	+	+	+		307 - 324		100.00
3*	XM_643009	XP_648101	364	+	+	+	+	+	+				100.00
4*	XM_648115	XP_653207	80	-	-	-	-	-	-	Th**** 50%: 14 - 24			100.00
5*	XM_643380	XP_648472	400	+	+	+	+	+	+	Th 50%: 150 - 175 262 - 265	327 - 350 372 - 393		20.00
6*	XM_643637	XP_648729	414	+	+	+	+	+	+	Th 50%: 152 - 174 203 - 265	334 - 360 386 - 406		20.00
7	XM_643379	XP_648471	308	+	+	+	+	+	+		284 - 305		16.67
8	XM_644114	XP_649206	309	+	+	+	+	+	+		285 - 306		12.50
9	XM_642923	XP_648015	306	+	+	+	+	+	+		283 - 305		11.11
10*	XM_645021	XP_650113	364	+	+	+	+	+	+				11.11
11	XM_643798	XP_648890	247	-	-	-	+	+	+		211 - 232		10.00
12*	XM_649824	XP_654916	362	+	+	+	+	+	+				10.00
13*	XM_648140	XP_653232	359	+	+	+	+	+	+	Th 90%: 221 - 235			5.56
14*	XM_645223	XP_650315	407	+	+	+	+	+	+	Th 90%: 302 - 313	324 - 345 351 - 369		4.76
15	XM_643164	XP_648256	304	+	+	+	+	+	+				3.57
16*	XM_643099	XP_648191	180	+	+	+	+	+	-				2.70
17	XM_643163	XP_648255	324	+	+	+	+	+	+				2.94
18	XM_643194	XP_648286	314	+	+	+	+	+	+		290 - 311		2.63
19	XM_643240	XP_648332	294	+	+	+	+	+	+				2.63
A = B
20*	XM_643464	XP_648556	304	+	+	+	+	+	+			1.25
21*	XM_643462	XP_648554	289	+	+	+	+	+	+			1.22
22*	XM_642922	XP_648014	403	+	+	+	+	+	+		288 - 309 315 - 341 352 - 374	1.12
23	XM_643102 XM_001914071	XP_648194 XP_001914106	303	+	+	+	+	+	+	Th 90%: 227 - 251		1.06
24*	XM_642959	XP_648051	291	+	+	+	+	+	+			1.04
25	XM_643063	XP_648155	386	+	+	+	+	+	+	Th 90%: 148 - 167	286 - 313 316 - 341 348 - 372		1.92
26	XM_643097	XP_648189	298	+	+	+	+	+	+				1.89
27	XM_644113	XP_649205	198	+	-	+	+	+	+				1.79
28	XM_644115	XP_649207	383	+	+	+	+	+	+	Th 90%: 194 - 208 211 - 217	287 - 308 310 - 335 341 - 369		1.67
29	XM_643463	XP_648555	319	+	+	+	+	+	+				1.64
30*	XM_643100	XP_648192	328	+	-	+	+	+	+	Th 50%: 149 - 166			1.61
31	XM_643195	XP_648287	367	+	+	+	+	+	+	Th 90%: 150 - 169 196 - 233	286 - 313 315 - 336 341 - 365		1.54
32	XM_643795	XP_648887	315	+	-	+	+	+	+	Th 90%: 153 - 166			1.30
33	XM_643721	XP_648813	291	+	+	+	+	+	+	Th 50%: 195 - 206			1.27
34	XM_648158	XP_653250	178	+	+	+	+	+	-				1.01
n.d.
35	XM_643797	XP_648889	287	+	+	+	+	+	+
36	XM_643634	XP_648726	290	+	+	+	+	+	+
37	XM_001914268	XP_001914303	298	+	+	+	+	+	+
38	XM_643632	XP_648724	308	+	+	+	+	+	+		286 - 305
39	XM_001914588	XP_001914623	203	+	+	+	+	+	+
40	XM_001914580	XP_001914615	285	-	+	+	+	+	+		260 - 282
41	XM_001914532	XP_001914567	145	+	+	+	+	+	-
42	XM_643239	XP_648331	163	+	+	+	-	-	+
43	XM_001914473	XP_001914508	179	+	+	+	+	+	-
44	XM_001914282	XP_001914317	188	-	-	+	+	+	+
45*	XM_643794	XP_648886	250	+	+	+	+	+	+
46*	XM_643014	XP_648106	290	-	-	-	+	+	+
47*	XM_643378	XP_648470	289	+	+	+	+	+	+	Th 50%: 212 - 241

GTP-binding motifs: G1 = GxxxxGKS; G2 = SET; G3 = VIDTPGL; G4 and G5 = not present; Conserved domains: C1 = GL/I/VQG/AIV/II/LV/TL/MN; C2 = HVCIVWTKC; C3 = RSExEIERLI; n.d.: not determined; *Removed from NCBI; **Prediction of coiled-coil domains using Marcoil1.0 http://www.isrec.isb-sib.ch/webmarcoil/webmarcoilC1.html[28]
***Prediction of transmembrane domains (TM) using TMHMM Server http://www.cbs.dtu.dk/services/TMHMM
****Treshold (Th)

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ISSN: 1471-2164

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