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Table 5 Development of PVY-F14K infection during screening and response in selected accessions.

From: EcoTILLING in Capsicum species: searching for new virus resistances

Accession Species eIF4E/eIF(iso)4E proteins Mean symptom indexa Mean absorbance (A405)a % positive plantsc Responsed
    15 Db 30 D 45 D 60 D 15 D 30 D 45 D 60 D   
CDP06188 C. annuum AC 0 0 0 0 0.05 0.03 0.03 0.02 0 R
CDP09688 C. annuum B_CD 0 0 0 0 0.02 0.02 0.02 0.02 0 R
CDP01263 C. chinense BB 3.5 2.5 1.5 1 1.24 0.89 0.65 0.29 100 S
CDP04928 C. annuum BC 0 0 0.5 1 0.07 0.46 0.72 0.37 100 S
CDP01246 C. annuum BD 0.5 0.9 1.1 1.5 0.27 0.51 0.61 0.57 100 S
CDP08791 C. chacoense CE 2 2.5 3.5 3.5 0.85 1.81 1.73 1.79 100 S
CDP02521 C. chinense DB 0 0 0 0 0.04 0.03 0.02 0.02 0 R
CDP06433 C. annuum EC 0 0 0 0 0.03 0.02 0.02 0.05 0 R
CDP01135 C. annuum ED 0 0 0 0 0.03 0.02 0.01 0.01 0 R
CDP00614 C. baccatum FA 0 0 0 0 0.54 0.25 0.17 0.26 100 T
CDP05838 C. frutescens FB 0 1.6 1 0.5 0.76 0.67 0.52 0.97 100 S
CDP00624 C. baccatum FC 0 0.5 0.5 0 0.87 0.35 0.14 0.35 100 S
CDP06234 C. baccatum FC 0 0 0 0 0.04 0.03 0.02 0.03 0 R
CDP00620 C. annuum FD 0.9 1.9 2.3 1.9 0.64 1.01 1.37 1.12 100 S
CDP05436 C. frutescens GB 0 0.9 1.3 1 1.36 0.88 0.98 1.33 100 S
CDP00973 C. frutescens GC 0 0.8 1 0.8 1.21 0.66 1.39 1.09 100 S
CDP08407 C. chinense HB 0 0 0 0 0.01 0.01 0.01 0.03 0 R
CDP07700 C. chinense IB 0 0 0 0 0.26 0.22 0.03 0.03 60 T
CDP00590 C. pubescens JG 2 2.4 1 0.2 1.81 1.6 0.68 1.55 100 S
CDP04291 C. baccatum KA 0 0 0 0 0.03 0.04 0.03 0.02 0 R
CDP02320 C. baccatum KC 0 0 0 0 0.03 0.03 0.02 0.04 0 R
CDP05581 C. baccatum LC 0 0 0 0 1.64 1.13 0.76 0.32 100 T
CDP06360 C. baccatum MC 0 0 0 0 0.07 0.08 0.09 0.09 60 T
CDP07825 C. baccatum MF 0 0 0 0 0.43 0.41 1.61 1.32 100 T
CDP04865 C. baccatum NA 0 0 0 0 0.03 0.04 0.04 0.06 0 R
CDP07490 C. baccatum NC 0 0 0 0 0.05 0.02 0.02 0.03 0 R
CDP04131 C. baccatum NF 0 0 0 0 0.05 0.05 0.04 0.06 0 R
CDP09967 C. baccatum OA 0 0 0 0 0.04 0.04 0.02 0.02 0 R
CDP09334 C. baccatum OC 0 0 0 0 0.03 0.04 0.03 0.04 0 R
CDP01186 C. chinense PB 2.5 3 3 3.5 0.88 0.19 0.23 0.25 100 S
CDP05929 C. baccatum QA 0 0 0 0 0.03 0.02 0.02 0.01 0 R
CDP04710 C. baccatum QC 0 0 0 0 0.33 0.18 0.13 0.16 100 T
CDP06951 C. frutescens RB 0 0 0 0 1.44 0.77 1.21 1.34 100 T
CDP02198 C. annuum Control 0.7 1.7 1.5 1.9 0.41 0.34 0.33 0.32 100 S
CDP05523 C. annuum Control 2.1 2.8 3.2 3.5 0.88 0.6 0.7 1.27 100 S
  1. a, Arithmetic mean calculated from all plants of the accession at each time sampling. The symptom index ranged from 0 (symptomless plant) to 4 (dead plant). b, D: Days post inoculation. c, Percentage of DAS-ELISA positive plants. Plants were considered to be DAS-ELISA positive when absorbance (405 nm) of sample from the youngest leaf was higher than three times the negative control mean, and classified as non-infected (DAS-ELISA negative) when the value of absorbance was below this threshold. The negative control mean was calculated for each accession with different time samplings (15, 30, 45 and 60 days post inoculation). d, R: Resistant. No plants were infected systemically and no symptoms were identified. S: Susceptible. Showed DAS-ELISA positive and systemic symptoms. T: Tolerant. Showed DAS-ELISA positive in all or some plants but without symptoms.