Figure 7

Histogram representing the G93A-SOD1 versus WT ratios of the spinal cord intensity values detected for Nfh, Map1b, S100A8, Timp2 and Hcn2 at 24 hours and at 7 days from compression injury, as measured by Bead-array and real-time RT-PCR analysis. The G93A-SOD1 vs WT expression ratios obtained using Bead-array (red code) and real time RT-PCR (blue code) analyses for Nfh, Map1b, S100A8, Timp2 and Hcn2 at two time points after compression SCI are reported. The two techniques of gene expression analysis confirm independently the same type of differential regulation for the selected gene candidates. S100A8 real-time RT-PCR confirms both the significant up-regulation in the G93A-SOD1 spinal cord detected by Bead-array analysis 7 days from compression SCI. Map1b RT-PCR has been included in order to validate the sensitivity of the real time RT-PCR and Bead array analyses for expression changes close to two-folds.