Skip to main content

Table 1 Main characteristics of the antibodies used for the experiments detailed in the manuscript.

From: The human G93A-SOD1 mutation in a pre-symptomatic rat model of amyotrophic lateral sclerosis increases the vulnerability to a mild spinal cord compression

  Manufacturer Product & Lot No. Host species Immunogen Dilution used
Primary antibodies     
Neurofilament (clone N52) Sigma N0142 & 65K4804 mouse C-terminal segment of enzymatically dephosphorylated pig neurofilament 200 1:20,000 using immunoperoxidase staining 1:1500 using Western blotting
Synaptophysin (clone SVP-38) Sigma S5768 & 90K4844 mouse Rat retina synaptosome 1:5000 using immunoperoxidase staining
Rat CD68- macrophages/monocytes (clone ED-1) Chemicon MAB1435 & 0606032963 mouse A single chain glycoprotein of 90-100 KDa that is expressed on the lysosomal membrane of myeloid cells 1:400 using immunofluorescent staining
Rat CD11b; RPE (clone OX-42) Serotec MCA275EL & 0105 mouse Rat peritoneal macrophages 1:200 using immunofluorescent staining
Glial fibrillary acid protein (clone SVP-38) Dako Z0334 & 00045904 rabbit Cow spinal cord 1:1000 using Immunofluorescent staining
β-actin (clone AC-15) Sigma A1978 & 118K4827 mouse Fusion of mouse myeloma cells and splenocytes immunized with a synthetic b-cytoplasmic actin N-terminal peptide 1:5000 using Western blotting
NeuroTrace (fluorescent Nissl stain) Molecular Probes N21480 & 4971-21 - - 1:100 according to manufacturer's instruction
Secondary antibodies     
Alexa Fluor 488 Invitrogen A21202 & 536050 donkey Anti-mouse IgG (H+L) 1:1000 using immunofluorescent staining
Alexa Fluor 568 Invitrogen A10042 & 538952 donkey Anti-rabbit IgG (H+L) 1:1000 using immunofluorescent staining
Alexa Fluor 680 Invitrogen A21057 & 65E2-1 goat Anti-mouse IgG (H+L) 1:15,000 using Western blotting