HR was overexpressed without prominent morphological changes in the skin of HrHp/HrHp and +/+ mice. (A-F) Cross-sections of the skin of hairpoor mice (HrHp) during early stages (E18.5, P0, and P3). At E18.5 (A, B) and P0 (C, D), noticeable differences were not found between +/+ and HrHp/HrHp skins. At P3 (E, F), There were shorter HFs detected in the HrHp/HrHp skin. HF did not grow deep into the subcutis layer in HrHp/HrHp skin than in the +/+. Scale bar = 100 μm. (G) Shape of hair bulbs in the +/+ and HrHp/HrHp skins at P3. (H) HRoverexpression in the skin of HrHp/HrHp mice at P0 and P3. Western blot analysis was performed with protein extracts from the backsides of mice at P0 and P3 using an HR antibody. The α-tubulin indicates equal amount of protein loading.