Differential expression of the genes of interest during HF development with real-time PCR. Four genes showed a consistent pattern of expression corresponding to Hr overexpression. Wif1 and Casp14 were upregulated (A) whereas Sfrp1 and Krt71 were downregulated (B) throughout the developmental stages. The remaining genes (Wnt7b, Jak2, Krt15, and Fgf10) displayed a complex expression pattern (C) during developmental stages (P0-P14). The Y-axis indicates the fold difference in expression, showing the relative expression level of each gene in HrHp/HrHp mice compared with the skin of age-matched +/+ mice. The values are the average of the relative expression level of three mice, each measured in duplicate.