Table 1 Sequencing and target enrichment results for 8 samples in uniplex, duplex and pentaplex reactions.
From: Combining target enrichment with barcode multiplexing for high throughput SNP discovery
Sample | Plexity | Filtered Reads | Mapped to ChrX | Mapped to Target Region | % Mapped to X | % Mapped to Target | Fold Enrichment |
|---|---|---|---|---|---|---|---|
A1 | Uniplex | 16,403,360 | 4,044,511 | 3,564,834 | 25.6 | 22.5 | 97 |
A2 | Duplex | 2,035,467 | 730,156 | 666,572 | 36.9 | 33.7 | 170 |
B1 | Duplex | 11,833,641 | 2,655,666 | 2,313,476 | 23.2 | 20.2 | 85 |
A3 | Pentaplex | 3,037,781 | 1,131,596 | 1,036,600 | 38.1 | 34.9 | 180 |
B2 | Pentaplex | 3,204,352 | 1,230,630 | 1,129,857 | 39.3 | 36.0 | 190 |
C1 | Pentaplex | 3,122,661 | 1,220,017 | 1,123,547 | 40.1 | 36.9 | 196 |
D1 | Pentaplex | 3,687,146 | 1,944,667 | 1,778,223 | 53.8 | 49.2 | 324 |
E1 | Pentaplex | 3,078,897 | 1,832,550 | 1,696,950 | 60.6 | 56.1 | 427 |