Figure 5

Transcription analysis of carbon source-dependent regulation of 2488prp and xyn2 transcription. (A) Transcription of 2488prp under repressing conditions. The H. jecorina strain QM9414 was pre-cultured on glycerol and then transferred to MA media lacking a carbon source (NC) or containing 1% (w/v) glucose (G) or glycerol (GY), and incubated for 3 and 5 hours, respectively. (B) Transcription of 2488prp under growth conditions. Replacement of QM9414 was performed to 1% (w/v) glucose (G), xylose (XO) or xylan (XN) as sole carbon source and incubated 3, 5 and 24 hours, respectively. (C) Transcription of 2488prp under inducing conditions. Replacement of QM9414 was performed to 1% (w/v) glucose (G), 1.5 mM sophorose (SO) or xylobiose (XB), and incubated 5 hours. (D) Transcription of xyn2 under various carbon source conditions. The rates of transcription for the above mentioned (growth) conditions are summarized. The relative transcript levels are given on decade logarithmic scale (lg). The data presented are means of results from three independent experiments. Error bars indicate the standard deviations. The asterisk indicates the reference sample.