Table 2 Sequencing statistics for libraries sequenced by the Sanger method
Library code | No. reads (I) | No of 3' reads in (1) | No. reads in OakContigV1 assembly | Number of high quality sequences (2) | Sequencing success rate % (2)/(1) | Average length (bp) in (2) |
|---|---|---|---|---|---|---|
A | 10717 | 0 | 10317 | 10313 | 96.3% | 535 |
B | 9615 | 4711 | 6673 | 6669 | 69.4% | 517 |
C | 392 | 0 | 224 | 224 | 57.1% | 313 |
D | 184 | 0 | 110 | 110 | 59.8% | 377 |
E | 203 | 0 | 148 | 148 | 72.9% | 404 |
F | 2493 | 0 | 2305 | 2305 | 92.5% | 566 |
G | 1756 | 0 | 1604 | 1604 | 91.3% | 500 |
H | 18935 | 4747 | 12002 | 11989 | 63.4% | 505 |
I | 19195 | 4868 | 15424 | 15424 | 80.4% | 597 |
J | 9377 | 4491 | 8964 | 8964 | 95.6% | 589 |
K | 9578 | 0 | 8652 | 8649 | 90.3% | 620 |
L | 9500 | 0 | 8533 | 8525 | 89.8% | 575 |
M | 19685 | 513 | 18756 | 18753 | 95.3% | 583 |
N | 1700 | 0 | 1518 | 1518 | 89.3% | 509 |
O | 2129 | 0 | 1975 | 1975 | 92.8% | 534 |
P | 7513 | 0 | 7238 | 7237 | 96.3% | 712 |
Q | 1765 | 0 | 1589 | 1589 | 90.0% | 522 |
R | 1768 | 0 | 1507 | 1507 | 85.2% | 495 |
S | 10164 | 0 | 9951 | 9950 | 97.9% | 584 |
T | 9158 | 4279 | 8435 | 8433 | 92.1% | 604 |
Total | 145827 | 23609 | 125925 | 125886 | 86.4% | 575 |