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Table 2 Sequencing statistics for libraries sequenced by the Sanger method

From: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak

Library code No. reads (I) No of 3' reads in (1) No. reads in OakContigV1 assembly Number of high quality sequences (2) Sequencing success rate % (2)/(1) Average length (bp) in (2)
A 10717 0 10317 10313 96.3% 535
B 9615 4711 6673 6669 69.4% 517
C 392 0 224 224 57.1% 313
D 184 0 110 110 59.8% 377
E 203 0 148 148 72.9% 404
F 2493 0 2305 2305 92.5% 566
G 1756 0 1604 1604 91.3% 500
H 18935 4747 12002 11989 63.4% 505
I 19195 4868 15424 15424 80.4% 597
J 9377 4491 8964 8964 95.6% 589
K 9578 0 8652 8649 90.3% 620
L 9500 0 8533 8525 89.8% 575
M 19685 513 18756 18753 95.3% 583
N 1700 0 1518 1518 89.3% 509
O 2129 0 1975 1975 92.8% 534
P 7513 0 7238 7237 96.3% 712
Q 1765 0 1589 1589 90.0% 522
R 1768 0 1507 1507 85.2% 495
S 10164 0 9951 9950 97.9% 584
T 9158 4279 8435 8433 92.1% 604
Total 145827 23609 125925 125886 86.4% 575
  1. Library codes are as in Table 1.