Table 3 SURF process summary
Library code | No. of reads (a) | No. match with chloroplast (b) | % match with chloroplast (b)/(a) | No. doubtful sequences (c) | % doubtful (c)/(a) | No. PCRkitful sequences (d) | % of PCRkit (d)/(a) | No. 'not valid' | % of 'Not valid' |
|---|---|---|---|---|---|---|---|---|---|
A | 10717 | 20 | 0.19% | 163 | 1.52% | 19 | 0.18% | 647 | 6.04% |
B | 9615 | 1 | 0.01% | 14 | 0.15% | 7 | 0.07% | 2951 | 30.69% |
C | 392 | 21 | 5.36% | 7 | 1.79% | 26 | 6.63% | 204 | 52.04% |
D | 184 | 19 | 10.33% | 1 | 0.54% | 31 | 16.85% | 100 | 54.35% |
E | 203 | 0 | 0.00% | 14 | 6.90% | 34 | 16.75% | 76 | 37.44% |
F | 2493 | 7 | 0.28% | 3 | 0.12% | 5 | 0.20% | 207 | 8.30% |
G | 1756 | 5 | 0.28% | 0 | 0.00% | 5 | 0.28% | 164 | 9.34% |
H | 18935 | 5 | 0.03% | 25 | 0.13% | 1 | 0.01% | 6976 | 36.84% |
I | 19195 | 4 | 0.02% | 28 | 0.15% | 5 | 0.03% | 3815 | 19.87% |
J | 9377 | 64 | 0.68% | 15 | 0.16% | 4 | 0.04% | 518 | 5.52% |
K | 9578 | 3 | 0.03% | 24 | 0.25% | 10 | 0.10% | 955 | 9.97% |
L | 9500 | 2 | 0.02% | 19 | 0.20% | 3 | 0.03% | 989 | 10.41% |
M | 19685 | 48 | 0.24% | 862 | 4.38% | 3 | 0.02% | 1894 | 9.62% |
N | 1700 | 7 | 0.41% | 0 | 0.00% | 9 | 0.53% | 191 | 11.24% |
O | 2129 | 4 | 0.19% | 1 | 0.05% | 3 | 0.14% | 173 | 8.13% |
P | 7513 | 12 | 0.16% | 194 | 2.58% | 28 | 0.37% | 492 | 6.55% |
Q | 1765 | 84 | 4.76% | 1 | 0.06% | 12 | 0.68% | 265 | 15.01% |
R | 1768 | 37 | 2.09% | 2 | 0.11% | 4 | 0.23% | 306 | 17.31% |
S | 10164 | 5 | 0.05% | 160 | 1.57% | 19 | 0.19% | 688 | 6.77% |
T | 9158 | 54 | 0.59% | 22 | 0.24% | 1 | 0.01% | 820 | 8.95% |
Total | 145827 | 402 | 0.28% | 1555 | 1.07% | 229 | 0.16% | 22431 | 15.38% |