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Table 3 SURF process summary

From: Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak

Library code No. of reads (a) No. match with chloroplast (b) % match with chloroplast (b)/(a) No. doubtful sequences (c) % doubtful (c)/(a) No. PCRkitful sequences (d) % of PCRkit (d)/(a) No. 'not valid' % of 'Not valid'
A 10717 20 0.19% 163 1.52% 19 0.18% 647 6.04%
B 9615 1 0.01% 14 0.15% 7 0.07% 2951 30.69%
C 392 21 5.36% 7 1.79% 26 6.63% 204 52.04%
D 184 19 10.33% 1 0.54% 31 16.85% 100 54.35%
E 203 0 0.00% 14 6.90% 34 16.75% 76 37.44%
F 2493 7 0.28% 3 0.12% 5 0.20% 207 8.30%
G 1756 5 0.28% 0 0.00% 5 0.28% 164 9.34%
H 18935 5 0.03% 25 0.13% 1 0.01% 6976 36.84%
I 19195 4 0.02% 28 0.15% 5 0.03% 3815 19.87%
J 9377 64 0.68% 15 0.16% 4 0.04% 518 5.52%
K 9578 3 0.03% 24 0.25% 10 0.10% 955 9.97%
L 9500 2 0.02% 19 0.20% 3 0.03% 989 10.41%
M 19685 48 0.24% 862 4.38% 3 0.02% 1894 9.62%
N 1700 7 0.41% 0 0.00% 9 0.53% 191 11.24%
O 2129 4 0.19% 1 0.05% 3 0.14% 173 8.13%
P 7513 12 0.16% 194 2.58% 28 0.37% 492 6.55%
Q 1765 84 4.76% 1 0.06% 12 0.68% 265 15.01%
R 1768 37 2.09% 2 0.11% 4 0.23% 306 17.31%
S 10164 5 0.05% 160 1.57% 19 0.19% 688 6.77%
T 9158 54 0.59% 22 0.24% 1 0.01% 820 8.95%
Total 145827 402 0.28% 1555 1.07% 229 0.16% 22431 15.38%
  1. Library codes are as in Table 1.