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Table 2 Accuracy and precision of platforms across linear range

From: Evaluation of external RNA controls for the standardisation of gene expression biomarker measurements

  Platform
Standards Microarray RT-qPCR
  Slope R2 Slope R2
13 1.0566 0.9991 1.0004 0.9988
42 1.0047 0.9675 1.0205 0.9988
81 1.0389 0.9671 0.9946 0.9994
84 0.9772 0.9639 0.9693 0.9989
95 0.957 0.9712 1.0022 0.9993
99 1.0095 0.9626 1.0052 0.9994
113 1.0248 0.9993 1.0200 0.9970
171 1.0696 0.9993 0.9714 0.9988
  1. The accuracy and precision of the platforms across the linear range (Microarray: 10-105 copies/ng; RT-qPCR: 10-106 copies/ng) were assessed by the slope (ideal slope = 1.000) and Pearson correlation coefficient (R2) values respectively, based on linear regression of transcript copies/ng vs. normalised signal intensity (microarray) or ΔCt values (RT-qPCR). RT-qPCR values were adjusted for the PCR efficiency of the assay.