Skip to main content

Table 2 Accuracy and precision of platforms across linear range

From: Evaluation of external RNA controls for the standardisation of gene expression biomarker measurements

 

Platform

Standards

Microarray

RT-qPCR

 

Slope

R2

Slope

R2

13

1.0566

0.9991

1.0004

0.9988

42

1.0047

0.9675

1.0205

0.9988

81

1.0389

0.9671

0.9946

0.9994

84

0.9772

0.9639

0.9693

0.9989

95

0.957

0.9712

1.0022

0.9993

99

1.0095

0.9626

1.0052

0.9994

113

1.0248

0.9993

1.0200

0.9970

171

1.0696

0.9993

0.9714

0.9988

  1. The accuracy and precision of the platforms across the linear range (Microarray: 10-105 copies/ng; RT-qPCR: 10-106 copies/ng) were assessed by the slope (ideal slope = 1.000) and Pearson correlation coefficient (R2) values respectively, based on linear regression of transcript copies/ng vs. normalised signal intensity (microarray) or ΔCt values (RT-qPCR). RT-qPCR values were adjusted for the PCR efficiency of the assay.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us