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Table 2 A comparison of the performance between the Rnnotator assembly and a single Velvet assembly.

From: Rnnotator: an automated de novo transcriptome assembly pipeline from stranded RNA-Seq reads

  Rnnotator (non-stranded) Rnnotator Velvet Oases Multiple-k
C. albicans SC5314      
   ▪ Accuracy1 94.0 95.0 97.4 92.3 96.6
   ▪ Completeness2 81.9 80.4 66.7 79.9 85.9
   ▪ Contiguity3 58.4 58.0 46.6 47.9 37.3
   ▪ Gene fusions4 1.73 0.26 1.18 1.31 0.20
C. albicans WO1      
   ▪ Accuracy 92.8 94.6 96.6 89.1 96.0
   ▪ Completeness 82.9 82.2 74.0 82.1 88.2
   ▪ Contiguity 59.1 59.4 43.3 48.6 48.7
   ▪ Gene fusions 2.06 0.65 1.38 1.61 0.46
  1. 1Accuracy is defined by the percentage of contigs that share at least 95% identity with the reference genome;
  2. 2Completeness is the percentage of known genes covered by the contigs to at least 80% of the gene length;
  3. 3Contiguity is the percentage of complete genes covered by a single contig over at least 80% of the gene length.
  4. 4Gene fusions are the percentage of contigs that contain more than 50% of two or more annotated genes.