Figure 4

Role of the ERSE motif in mouse CRELD2 and ALG12 promoter activities in Neuro2a cells. A) The nucleotide sequence of a common ERSE motif in the mouse CRELD2-ALG12 gene is shown. NF-Y and ATF6 binding sequences in the ERSE motif and their mutated sequences are shown with bold types and small letters, respectively. Neuro2a cells were transfected with the indicated CRELD2 or ALG12 reporter constructs. Twenty-four hours after transfection, the cells were incubated with or without Tg (0.1 μM) for 10 h (B and C). After incubation, the cells were lysed and luciferase activity was measured as described in methods. The activities obtained from control and Tg-treated cells are shown as open and closed columns, respectively. Values represent means ± SD from 3 - 4 independent cultures and are expressed relative to the basal activity of the pGL3-Basic.