Figure 8

Characterization of the promoter activity of the full intergenic region of the mouse CRELD2 - ALG12 gene pair. A) Neuro2a cells were transfected with the indicated CRELD2 or ALG12 reporter constructs. Twenty-four hours after transfection, the cells were incubated with or without Tg (0.1 μM) for 10 h, and the luciferase activity was measured. B) The expression vector for ATF6 or an empty vector (mock) was co-transfected with each reporter construct into Neuro2a cells. Thirty-six hours after transfection, luciferase activity for each construct was measured. Control and Tg- or ATF6-stimulated activities are shown as open and closed columns, respectively. Values represent means ± SD from 3 independent cultures and are expressed relative to the basal activity of the pGL3-Basic vector.