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Figure 4 | BMC Genomics

Figure 4

From: Structure and dynamics of the operon map of Buchnera aphidicola sp. strain APS

Figure 4

Experimental testing of B. aphidicola gene pair status (STU or DTUs), by RT-PCR amplification using specific primers (third lane). For each gene pair amplification we used a positive control, in which gDNA was used instead of cDNA for the PCR reaction (first lane) and a negative control, for which the reverse transcriptase was omitted from the RT reaction (second lane). The size (bp) of the amplicon is shown below each gene pair. (a) gene pairs predicted as STU solely by DisTer; (b) gene pairs predicted as STU by DisTer with no consensual annotation given by the 3 other methods; (c) gene pairs predicted as STU by all methods; (d) gene pairs predicted as DTUs by DisTer; (PC) positive controls; (NC) negative controls.

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