Figure 1
From: Apoplast proteome reveals that extracellular matrix contributes to multistress response in poplar
Assessment of symplastic contamination of leaf apoplastic extracts. (A) Light microscopy images from pressured and control P. deltoides leaves. S, stomata; vb, vascular bundle. Bar = 25 μm. (B) Protein distribution on 2-D PAGE from the extracts of leaf apoplast and whole leaf tissue. rbcL, the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit. (C) Protein distribution on 1-D SDS PAGE and immunoblot analysis of symplastic rbcL and cytosolic malate dehydrogenase (cMDH) using the extracts from apoplast and whole leaf tissue. kDa, protein molecular weights. (D) Macro and (E) micro nutrients in leaf apoplast and whole leaf extracts. Asterisks indicate statistically significant (p < 0.001) difference in nutrients between apoplast and whole leaf extracts. Ppm, parts per million.