Figure 4

Venn diagram of the final result lists of genes that are differentially spliced in NSCLC compared to NAT. From the primary result list of the enhanced workflow (see Figure 1) sub-lists were generated in order to inspect gene expression profiles manually. Genes shown were selected for validation using independent laboratory methods. Sub-list A: Genes that showed evidence for differential splicing and that were not affected by differential gene expression (top 100). Sub-list B: Genes that showed evidence for differential splicing and that were affected by differential gene expression (top 100). Sub-list C: Genes with exons that are known to be involved in alternative splicing and where these exons showed a high splicing index (SI ≤ 1.4^-1 or SI ≥ 1.4).