Figure 7

Alternative splicing of FOX2 affecting exons in the C-terminal region. (a) Exon structure (green: constitutive exons; orange: annotated cassette exons; purple: putative cassette exon predicted by orthology), location of RT-PCR primers (green arrows), and unique restriction enzyme cleavage sites (exon and primer sizes are shown to scale, introns not to scale). (b) Verification of RT-PCR product sizes generated from paired samples of adenocarcinoma of NSCLC and NAT of six patients (ø: no template control). (c) RT-PCR product sizes analysed using the Bioanalyzer. (d) Cleavage by Hpy8I indicated presence of the first cassette exon. (e) No cleavage by AfeI indicated absence of the second cassette exon. (f) No cleavage by HinfI indicated absence of the third cassette exon.