Advertisement
Table 4 Mapping statistics for sea bass BAC-ES comparatively mapped to the stickleback chromosomes XV to XXI.
| A | stickleback chromosome | CHR XV | CHR XVI | CHRXVII | CHRXVIII | CHRXIX | CHR XX | CHR XXI |
|---|---|---|---|---|---|---|---|---|
| stickleback chromosome size [bp] | 16,198,764 | 18,115,788 | 14,603,141 | 16,282,716 | 20,240,660 | 19,732,071 | 11,717,487 | |
| genetic linkage group sea bass | LG13 | LG15 | LG1? | LG17 | LG6 | LG16/18? | LG18? | |
| B | sea bass BAC-contigs | 20 (29) | 24 (38) | 30 (30) | 35 (43) | 38 (43) | 22 (35) | 9 (17) |
| largest BAC-contig [bp] | 3,426,120 (1,723,928) | 6,616,511 (2,444,746) | 3,516,936 (2,019,946) | 1,934,982 (1,280,086) | 2,450,075 (2,125,025) | 7,220,725 (2,296,377) | 4,139,228 (2,305,187) | |
| % of chr. covered by BAC-contigs | 89.3% (82.5%) | 88.4% (79.0%) | 88.5% (78.7%) | 83.6% (70.6%) | 86.6% (78.2%) | 90.3% (82.8%) | 87.4% (83.3%) | |
| number of BACs in min. tiling path | 156 (150) | 182 (167) | 163 (138) | 170 (143) | 206 (185) | 194 (178) | 111 (116) | |
| paired end aligned consistent BACs | 969 (619) | 916 (533) | 784 (459) | 783 (373) | 1,004 (628) | 1,077 (639) | 639 (393) | |
| one end aligned BACs | 1,017 (1,057) | 1,163 (1,162) | 1,110 (1,145) | 989 (1,040) | 1,204 (1,187) | 1,178 (1,183) | 693 (691) | |
| C | inconsistently aligned same chr. | 73 (96) | 47 (74) | 37 (34) | 68 (78) | 104 (128) | 30 (46) | 7 (10) |
| potential intra-chr. rearrangements between stickleback and sea bass | 10 | 8 | 6 | 12 | 14 | 8 | 2 | |
| intra-chr. rearrangements also found between medaka and stickleback | 9 | 4 | 4 | 6 | 8 | 6 | 2 | |
| BAC-contigs with same neighbour in medaka as in stickleback | 3 | 7 | 4 | 9 | 7 | 3 | 4 | |
| D | total annotated genes in ensembl | 779 | 799 | 698 | 761 | 1,037 | 927 | 460 |
| % genes covered by BAC-contigs | 87.9% (84.1%) | 88.5% (80.4%) | 85.5% (77.4%) | 82.7% (69.5%) | 82.5% (75.5%) | 89.5% (85.1%) | 93.9% (86.7%) |
