Figure 4From: Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3RT-PCR confirmation of microarray data. RT-PCR was performed using RNA from unstressed (-) or drought stressed (+) control and 35S:ABF3 plants at either the 2 h or 24 h time point. Genes showing either enhanced or attenuated regulation in 35S:ABF3 plants in response to drought were amplified using gene-specific primers. Elongation factor 1-α (EF-1) was amplified as an internal control.Back to article page