Figure 4

Pax7 expression depends on Pax3. (A, B) Whole mount in situ hybridisation with a Pax7 probe of wild-type (WT) (A) and Pax3^Pax3-En/+ partial loss of function (B) embryos at E10.5, showing down-regulation of Pax7 transcripts when Pax3 activity is reduced. (C) GFP positive cells were isolated by flow cytometry (see Figure 2) from the interlimb somites of Pax3^GFP/+ and Pax3^GFP/nlacZ embryos at E10.5. The same number of cells in each case was used for RNA preparation and quantitative PCR analysis, with Pax7 and Gapdh primers; mRNA levels are shown relative to Gapdh transcripts with the heterozygote control level taken as 1. Histograms represent mean fold change for four different biological samples analyzed in duplicate at each stage; error bars, standard error to the mean (SEM); **p < 0.005.