Comparison and variability analysis of NILTs from Atlantic salmon. a. Multiple sequence alignment of the deduced amino acid sequences of Ssa-NILT1-6 using Clustal W. Conserved cysteines are indicated in black. The transmembrane region is highlighted in gray. Putative inhibitory signalling motifs (ITIMs) in the cytoplasmic region are underlined. The predicted eight beta-strands are denoted by asterisks. LP, leader peptide; CP, connecting peptide; TM, transmembrane region; Cyt, cytoplasmic region. Dots (.) indicate identity to Ssa-NILT1 and dashes (-) denote gaps introduced for optimal alignment. b. Analysis of the variability of the entire Ssa-NILT protein sequences using Shannon entropy calculations. Positions with a Shannon entropy higher than 1.3 are considered significantly variable (indicated by black line).