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Table 3 List of primers used for quantitative real-time PCR and sequencing

From: Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential

Target gene

Forward primer

Reverse primer

glyA

CAAGCGAATGCAGCTGTTTA

CTGTGATGCCGTAGAGGACA

pykA

GTACGGATGCGGTAATGCTT

GTACGGATGCGGTAATGCTT

tpiA

CTACGAACCGATTTGGGCTA

CCGCCGTATTGGATAATCAC

exbB1

CCGGAATTGCGACAAATAGT

CCGTTCATTGGGTTATTTGG

tonB1

CATTGCATTGCCATAACCAG

AAAAGCGCCTGAAAAGATCA

tbpA1

AGGAATGACGTTGGTTTTGC

ATTGCAGGTAGGGCTGATTG

copA

CTATGAAGCCAGCGTGATGA

CCAAATAGAACCGCTTTCCA

tonB2

GCCTTGTACCGCATTAGGAA

CTCAGCCTAAGCCGAAAGAA

hlyX

TTTTACGTTGAGCGAACACG

ACGCCGTAATTTGTTCTTCG

cirA

TACGCTCTCCGGTGTGTATG

GTTGCGGTAGAAGCACCTTT

ywbN

TCGCAAATGGGCTTTAATTC

CTTTCAGCCAACCGTCTTGT

hpuB (APL_1953)

AACATCGTGTAAGCGCCTCT

GCCCTCATCATCGGTATCAC

hpuB (APL_1954)

ACGAAATGTTCTCCGGTACG

GATAGCCGGTCGAAACGTAA

hpuB (APL_1955)

GGTTCGGCAACCTTATTTGA

CGTTCTAACCCGCGTAATTC

lldD

AATGCCCTTGATTACCATCG

GTAAACCGCATACGCTTGGT

hyaA

TTTACCGGGTATGCCGATTA

GTGTCCTTCATCGCCGTATT

hybB

TAATACCGGCAAAGGCTGTC

ACTTTCGCAAACTCGCCTAA

napB

ACCCGTCGTGCTTGATACTT

GGCTTATACCAACCCGCATA

nqrC

CCGTAGCTAAAGGTGCTTCG

TTAGCTCCCATTGCTGCTTT

fdxG

TACTGTTCTGTCGGCTGTGG

GACTTACCGGATGGTCAGGA

ykgE

GTTTAAACGACCGGCAATGT

AACAAACCTGTTGCGGTTTC

hpuB (APL_1954)*

TCGGAGGAAAACTCGCTTTA

TAACCACCGGTCGGAAAATA

  1. All primers were designed by Primer3 http://frodo.wi.mit.edu/primer3/ and purchased at DNA Technology A/S, Risskov, Denmark. *Primers used for sequencing.
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BMC Genomics

ISSN: 1471-2164

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