Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential

Table 4 Relative expression results from REST analysis of qPCR data

No.	Gene	Type	Reaction Efficiency	Expression	Std. Error	P(H1)*
	glyA	Reference	0.91	1.33
	pykA	Reference	0.99	1.00
	tpiA	Reference	0.99	0.75
1	exbB1	Sample	1.00	13.94	4.26 - 35.88	0.000
2	APL_1953	Sample	0.99	6.82	2.45 - 20.84	0.000
3	APL_1954	Sample	0.98	5.09	1.49 - 16.29	0.000
4	APL_1955	Sample	1.00	6.68	2.44 - 20.64	0.000
5	cirA	Sample	0.85	2.26	0.92 - 5.30	0.001
6	ywbN	Sample	1.00	3.76	1.87 - 7.70	0.000
7	copA	Sample	1.00	6.98	2.79 - 17.86	0.000
8	hlyX	Sample	0.95	1.78	0.70 - 5.14	0.017
9	tbpA1	Sample	0.90	7.82	2.62 - 18.50	0.000
10	tonB1	Sample	0.94	12.60	3.56 - 34.29	0.000
11	tonB2	Sample	1.00	1.65	0.75 - 3.66	0.012
12	lldD	Sample	0.98	15.32	1.69 - 90.73	0.000
13	napB	Sample	0.94	0.18	0.07 - 0.58	0.000
14	nqrC	Sample	0.93	0.36	0.18 - 0.74	0.000
15	ykgE	Sample	0.89	0.12	0.04 - 0.45	0.000
16	hyaA	Sample	1.00	0.05	0.02 - 0.21	0.000
17	fdxG	Sample	1.00	0.30	0.07 - 1.37	0.004
18	hybB	Sample	0.93	0.09	0.03 - 0.37	0.000

*The hypothesis test represents the probability of the alternative hypothesis that the difference between the sample and control groups is due only to chance. Results are based on 6000 randomizations.

ISSN: 1471-2164