Figure 1

Microscopical examination of the KA-defect. A. Confocal laser scanning microscopy images of spermatozoa with acrosomal granule from two KA-affected boars. Yellow lines indicate the perpendicular planes of the 3d-reconstructions. Arrows indicate a vacuolum inside the granule. B. TEM analysis of an acrosomal granule. Two vacuoles are present and the apical part of the nucleus shows a Y-shape.