Figure 1
From: Decoding pooled RNAi screens by means of barcode tiling arrays
shRNA expression construct. Expression of shRNAs from pGIPZ vector constructs is driven by an RNA Polymerase II promoter (CMV). Each shRNA template is associated with a unique 60 nt barcode sequence. Every barcode sequence can be amplified by one primer pair from a pool of constructs. For analysis of pooled RNAi screens, six overlapping tiling probe sequences (25 nt) complementary to each barcode were synthesized on a Geniom One microarray. puror, puromycin resistance gene; shRNA, shRNA template; White areas indicate common sequence among all shRNA expression constructs.