Figure 2

Comparison between half hairpin and barcode tiling analysis. Shown are the histograms of probe signal intensities from reference arrays of half hairpin (A) and barcode tiling probes (B) as multiples of the median background intensity. Dashed lines represent a 4-fold median background intensity threshold. Normalized (test/reference) ratios are plotted against the indicated dilution factors in linear (top) and logarithmic scale (bottom) for analysis via half hairpin (C) and barcode tiling probes (D). Values for (test/reference) ratios, standard deviations, p-values and number of analyzable constructs per dilution step are shown in Table 2.