Figure 4

Overview of results from negative selection screens. A - The log₂ (t_end/t_zero) signal intensity ratios were calculated from all probes that passed the described filter criteria and averaged for each shRNA expression construct. Negative log₂ ratios indicate the depletion of cells expressing a particular shRNA from the pool of cells, following the four weeks of the screen. B - The log₂ ratios determined for each shRNA expression construct were plotted against their significance. The red dashed line represents a p-value of 0.05 which was used as cut off. Highlighted in red are significant candidate shRNAs (numbers) and validated candidate constructs. The indicated numbers correspond to the numbers given in the Additional file 2.