Figure 5
From: Decoding pooled RNAi screens by means of barcode tiling arrays
Validation assays of individual candidate constructs. A - Shown are the (tend/tzero) ratios from the pooled screen, the target gene expression relative to the NSC at six days post-infection, the cell viability relative to the NSC at eight days post-infection, and the activity of caspase 3/7 relative to NSC as a measure of apoptosis induction at six days post infection. NSC, non silencing control. B - Shown are the (tend/tzero) ratios determined over four weeks of the pooled screen, together with the cell viability relative to the NSC at eight and sixteen days post-infection. The inhibition of HSPA8 leads to a more pronounced decrease in viability at sixteen days as compared to eight days post infection, in accordance with the (tend/tzero) ratios. NSC, non silencing control.