Table 2 Comparison of half hairpin and barcode tiling analysis
From: Decoding pooled RNAi screens by means of barcode tiling arrays
Half hairpin probes | Barcode tiling probes | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Dilution factor | Mean ratio [test/ref.] | σ | p -value | n | Dilution factor | Mean ratio [test/ref.] | σ | p -value | n | |
1e-06 | 0.04 | 0.06 | 8e-28 | 7 | 1e-06 | 0.02 | 0.02 | 4e-80 | 10 | |
1e-05 | 0.14 | 0.19 | 7e-28 | 6 | 1e-05 | 0.04 | 0.02 | 4e-77 | 9 | |
1e-04 | 0.19 | 2 | 1e-04 | 0.04 | 0.01 | 5e-67 | 9 | |||
2e-04 | 0.05 | 0.09 | 8e-19 | 4 | 2e-04 | 0.02 | 0.01 | 3e-90 | 9 | |
1e-03 | 0.37 | 0.18 | 1e-09 | 4 | 1e-03 | 0.04 | 0.03 | 5e-75 | 10 | |
2e-03 | 0.03 | 0.04 | 5e-23 | 4 | 2e-03 | 0.02 | 0.01 | 4e-70 | 8 | |
4e-03 | 0.11 | 0.08 | 7e-21 | 4 | 4e-03 | 0.06 | 0.04 | 7e-80 | 9 | |
1e-02 | 0.16 | 0.12 | 6e-33 | 7 | 1e-02 | 0.07 | 0.01 | 8e-80 | 10 | |
4e-02 | 0.31 | 0.09 | 2e-17 | 4 | 4e-02 | 0.20 | 0.05 | 7e-57 | 9 | |
1e-01 | 0.58 | 0.15 | 7e-11 | 6 | 1e-01 | 0.31 | 0.09 | 9e-40 | 9 | |
2e-01 | 0.85 | 2 | 2e-01 | 0.49 | 0.09 | 6e-21 | 9 | |||
3e-01 | 0.88 | 0.08 | 3e-01 | 4 | 3e-01 | 0.64 | 0.12 | 6e-17 | 10 | |
4e-01 | 0.94 | 0.10 | 7e-01 | 4 | 4e-01 | 0.74 | 0.04 | 1e-09 | 8 | |
5e-01 | 0.97 | 0.10 | 9e-01 | 7 | 5e-01 | 0.78 | 0.06 | 7e-08 | 10 | |
6e-01 | 0.83 | 0.23 | 6e-02 | 6 | 6e-01 | 0.85 | 0.05 | 3e-04 | 9 | |
7e-01 | 1.04 | 2 | 7e-01 | 0.88 | 0.09 | 9e-03 | 9 | |||
8e-01 | 0.97 | 0.08 | 1e+00 | 4 | 8e-01 | 0.96 | 0.09 | 5e-01 | 10 | |
9e-01 | 1.14 | 0.38 | 9e-02 | 4 | 9e-01 | 1.01 | 0.09 | 8e-01 | 8 | |
1e+00 | 1.00 | 0.18 | 1e+00 | 106 | 1e+00 | 1.00 | 0.12 | 1e+00 | 225 | |