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Figure 3 | BMC Genomics

Figure 3

From: Gene discovery in the horned beetle Onthophagus taurus

Figure 3

Bi-connected components and alternative splicing. A. An example of a bi-connected component structure (BCCs). A BBC composed of three contigs 28477, 25928 and 04341 that share three independent sets of 31, 28, and 8 broken reads, respectively (indicated by dashed line) in relation to the homologous T. castaneum transcript (Tc XM963744). Our analysis suggests that this pattern is reflective of two alternative splice variants present in the Onthophagus transcriptome. B. The three conceptual polypeptide sequences from these contigs align to a contiguous region of the Disabled protein from Tribolium, supporting this hypothesis. Shown are, from top to bottom, two alternative Drosophila Disabled transcripts (dark blue lines; thin light blue lines indicate first methionine (M) and stop codon (*)), the homologous Tribolium sequence (green; no alternative transcripts are known from Tribolium) and the relative positions of contigs 28477, 25928 and 04341. Note that the contig 28477 (light orange), which based on our analysis is a putatively alternatively spliced exon, does not share similarity with the exon that is alternatively spliced in Drosophila.

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