Figure 1

Colony organization of the P. vortex bacteria. (A) Whole colony view of P. vortex, when grown on 15 g/l peptone and 2.25% (w/v) agar for four days. The bright yellow dots are the vortices as described in the text. (B) Two colonies of P. vortex, inoculated in two parallel lines, on 15 g/l peptone and 2.25% (w/v) agar. Structure flexibility of the colony architecture is illustrated. The colonies in A and B were grown in a Petri dish size 8.8 cm and stained with Coomassie dyes (Brilliant Blue). The colors were inverted to emphasize higher densities using the brighter shades of yellow. (C) Magnification of x20 into the colony pattern and vortex progress. (D) An example of a mature individual vortex magnification x500. (E) Scanning electron microscope (SEM) observation of P. vortex illustrating a typical bacteria arrangement in the center of a vortex. Notable, that each individual bacterium has a curvature. Scale bar in (A-B) is 1 cm, in (C) is 500 μm, in (D) is 20 μm and in (E) is 5 μm.