From: Comparison of transcriptomic landscapes of bovine embryos using RNA-Seq
Sample | Blastocysts | Degeneratives |
---|---|---|
Paired end reads | 18,377,462 × 2 | 20,499,571 × 2 |
Single end reads | 17,597,407 | 13,770,885 |
Total sequenced fragments | 35,974,869 | 34,270,456 |
Fragments mapped to nuclear genome | 22,511,851 | 21,480,971 |
   (percent mapped) | (62.6%) | (62.7%) |
Uniquely mapped fragments | 20,417,798 | 19,830,476 |
   (percent uniquely mapped) | (90.7%) | (92.3%) |
Fragments mapped to mitochondrial genome | 3,690,752 | 2,188,146 |
Fragments mapped to annotated junctions | 2,174,923 | 1,268,315 |
   (number of junctions) | (65,743) | (62,788) |
Fragments mapped to putative novel junctions | 129,851 | 84,600 |
   (number of junctions supported by at least 2 fragments) | (10,638) | (8,686) |
Fragments mapped to annotated exons | 11,596,019 | 7,103,823 |
Fragments overlapped with annotated introns | 2,525,630 | 3,644,395 |
Fragments mapped to annotated genes (exons + introns) | 14,121,649 | 10,748,218 |
Fraction of intronic fragments among genic fragments | 17.9% | 33.9% |
PolyA containing reads | 534,090 | 862,915 |
Mapped polyA reads (with polyA signal) | 112,170 | 120,552 |
   (number of putative polyA sites) | (11,389) | (15,493) |