Figure 4

Splice scores compare well with RT-PCR data. Tissue-specific alternative splicing data are shown for four genes: FEZ2 (A), TPD52 (B), CAMK2D (C) and MINK1 (D). In each panel schematic representations of the analyzed splice junctions are shown on top. Exons are depicted as boxes and numbered; lines connecting the exons specify exon junctions; arrows show the position of PCR primers used for the RT-PCR gel electrophoresis splicing analysis. Below the schematics are graphs that plot splice scores for three sets of data: sequenced asMIPs (squares), array quantified asMIPs (diamonds) and qPCR (triangles). The M-score data are plotted for five different tissues: placenta (PLA), skeletal muscle (SKM), stomach (STM), cerebellum (CEB) and frontal lobe (FRL). Analyzed exon junctions are color-coded and specified to the right of each graph (e.g. "5-6" corresponds to data for the junction between exons five and six). Gray dashed lines show the M-score cutoffs for qPCR (+/-2.7) and asMIP data (+/-1.3). Tissue-specific RT-PCR, gel electrophoresis data is pictured below each splice-score graph. PCR primers surrounding the analyzed exon junctions were used to amplify cDNA reverse transcribed from RNA extracted from the specified tissues. A reference sample (REF) containing reverse-transcribed total RNA from >20 human tissues is pictured to provide a pictorial approximation of baseline splicing for comparison with the graph. The exons contained within each PCR gel band are specified to the right of each gel.