QPCR of selected genes with stress relevant functional annotations identified in the forward SSH libraries (designed to be enriched for transcripts up-regulated by heat-shock): Part I. The RQs (relative quantities), normalized to 18S ribosomal RNA expression and calibrated to the individual with the lowest expression of each gene of interest (see methods section), are presented as averages ± SEM. The levels of gene expression of heat-shock protein 90 alpha (HSP90α: A), heat shock protein 90kDa beta, member 1 (GRP94: B), HSP70-1 protein (HSP70-1: C), 78 kDa glucose regulated protein (GRP78: D), heat-shock protein 47 (HSP47: E) are shown for the control transferred (CT) and heat-shocked (HS) groups before heat-shock (BHS), at the cessation of heat-shock (CS), 3h after the cessation of heat-shock (3ACS), and 12h after the cessation of heat-shock (12ACS). Different letters indicate significant differences between sampling points within the same treatment (p<0.05). * indicates significant differences between CT and HS groups at a given sampling point (p<0.05). Numbers in the boxes represent overall fold-changes. For each treatment, overall fold upregulation was calculated relative to the appropriate before heat-shock (BHS) value as (average RQ of time point)/(average RQ of appropriate group at the BHS time point), and overall fold down-regulation was calculated when necessary (i.e. if overall fold upregulation was < 1) as the inverse of overall fold up-regulation. Each panel shows the expression for a given gene of interest in all 3 tissues studied. L: liver. HK: head kidney. M: skeletal muscle.