Primer name | Sequence | Gene name of the best BLASTx hit | Amplification efficiency | Amplicon size (bp) |
---|---|---|---|---|
HSP90α Forward | 5'- GAA CAA GAC CAA GCC CCT TT -3' | Heat shock protein 90 alpha | 107% | 133 |
HSP90α Reverse | 5'- CTG ACC CTC CAC CGA GAA GT -3' |  |  |  |
GRP94 Forward | 5'- AGT GTT TCT CTC GAC ACG TTC A -3' | Heat shock protein 90 kDa beta, member 1 (synonym: GRP94) | 91% | 97 |
GRP94 Reverse | 5'- CAG ACG ACT TCC ATG ACA TGA T -3' | Â | Â | Â |
HSP70-1 Forward | 5'- GAG AAC AAG ATC ACC ATC ACG A -3' | HSP70-1 (synonym: HSPA1A) | 97% | 123 |
HSP70-1 Reverse | 5'- GGC TGT TAC TTT CTC TCC CTG A -3' | Â | Â | Â |
GRP78 Forward | 5'- CTC CTT CAT TTT GGT CAG AAC C -3' | 78 kDa glucose-regulated protein (synonym: GRP78) | 90% | 132 |
GRP78 Reverse | 5'- CTC AAG TTC CTC CCA TTC AAA G -3' | Â | Â | Â |
HSP47 Forward | 5'- ATG GAA GTC AGC CAC AAC CT -3' | Heat Shock Protein 47 | 93% | 100 |
HSP47 Reverse | 5'- TCT TGC CCG TGA TGT TAG AC -3' | Â | Â | Â |
CCT1 Forward | 5'- GCA GGC GTT TGG GAT AAC TA -3' | T-complex-1 | 95% | 127 |
CCT1 Reverse | 5'- GCG CTT AAC CCT TCA GAG AA -3' | Â | Â | Â |
CCT5 Forward | 5'- CCA GGC GAG GTT GAA GAA TA -3' | Chaperonin containing TCP1, subunit | 92% | 91 |
CCT5 Reverse | 5'- TAG AAC AGG GGA GTG GTG GT -3' | 5 (epsilon) | Â | Â |
TCTP Forward | 5'- ACC AAG CCA GAG AGA GTG GA -3' | Translationally-controlled tumor protein | 101% | 147 |
TCTP Reverse | 5'- ATC CTC ACG GAA GTC AAG CA -3' | Â | Â | Â |
Enolase Forward | 5'- GGA CGG CAC TGA AAA CAA AT -3' | Enolase 3 | 93% | 115 |
Enolase Reverse | 5'- ACA GAG GAA CCC CCT TCT CC -3' | Â | Â | Â |
PFK Forward1 | 5'- TGT TTG CCA ACT CCC CAG AGA -3' | Phosphofructokinase, muscle a | 96% | 121 |
PFK Reverse1 | 5'- TCC GGT GCT TGA AGT CTG TCA -3' | Â | Â | Â |
Aldolase Forward1 | 5'- TGA CAT TGC TCA GAG GAT GG -3' | Aldolase B | 91% | 143 |
Aldolase Reverse1 | 5'- TAG CGA CGG TTC TCC TCA CT -3' | Â | Â | Â |
Bikunin Forward | 5'- GCC ACT GAG TTC ACA GAC G -3' | Alpha-1-microglobulin/bikunin precursor | 90% | 107 |
Bikunin Reverse | 5'- CAG CTC ATG GAG GAG GAG T -3' | Â | Â | Â |
IgM Forward | 5'- GAG CAT CCA CTG GCT CTT TA -3' | Immunoglobulin heavy chain, secretory form | 93% | 97 |
IgM Reverse | 5'- GCA GCA AGC TAT ATC CAG GT -3' | Â | Â | Â |
Nupr1 Forward | 5'- CTT TCT TCT CGC TGT TCT GC -3' | Nuclear protein 1 | 88% | 101 |
Nupr1 Reverse | 5'- GGA AGG ACC AAG AAG GAG TC -3' | Â | Â | Â |
IL-8 Forward | 5'- CTT CAG CAT CCA GAC AGA CC -3' | Interleukin-8 | 100% | 133 |
IL-8 Reverse | 5'- CAG ACA GAG AGC CGT CAG AT -3' | Â | Â | Â |
TLR22 Forward | 5'- TGC AGG TAA TCA CGA CTG AC -3' | TLR22 | 85% | 93 |
TLR22 Reverse | 5'- GAG ACT TCC AGC CAG ACC TA -3' | Â | Â | Â |
18S Forward | 5'- ATG GCC GTT CTT AGT TGG TG -3' | 18S ribosomal RNA (normalizer gene) | 109% | 180 |
18S Reverse | 5'- GGA CAT TTA AGG GCG TCT CA -3' | Â | Â | Â |