Figure 1

Annotation and analysis of the C. pseudotuberculosis FRC41 genome sequence. (A), Plot of the annotated C. pseudotuberculosis FRC41 chromosome. The circles represent from the outside: circle 1, DNA base position [kb]; circle 2, protein-coding regions transcribed clockwise; circle 3, protein-coding regions transcribed anticlockwise; circle 4, G/C skew plotted using a 10-kb window; circle 5, G+C content plotted using a 10-kb window. The protein-coding regions are coloured according to their functional classification into the Clusters of Orthologous Groups of proteins [111]. (B), Architecture imparting sequences in the C. pseudotuberculosis FRC41 chromosome. The distribution of the octamers G(A/T/C)GGGGGA and (T/C)GGGGGAG on the leading and lagging strands is shown. The origin of replication (oriC) is marked. The deduced dif locus is located at around 1.1 Mbp of the chromosomal map. The 28-bp sequence of the predicted dif region is shown. The location of the four rRNA operons (rrnA-rrnD) on the leading strands is indicated. (C), Synteny between the chromosomes of C. pseudotuberculosis FRC41 and C. diphtheriae NCTC 13129. The X-Y plot is composed of dots forming syntenic regions between both chromosomes. The dots represent predicted C. pseudotuberculosis FRC41 proteins having an orthologue in the genome of C. diphtheriae NCTC 13129 with co-ordinates corresponding to the position of the respective coding region in each genome sequence and indicated in kb. Orthologous proteins were detected by reciprocal best BLASTP matches.