Figure 3

Regulons involved in iron regulation of C. pseudotuberculosis FRC41. The DtxR and RipA regulons controlling iron homeostasis and the respective gene-regulatory interactions were deduced from a genome-scale network transfer approach [114] and the combined use of hidden Markov models and position weight matrices [74]. The assignment of the transcription regulators into the regulatory protein families of C. pseudotuberculosis FRC41 is indicated. Predicted DNA-binding sites are listed by sequence and are shown as black boxes, regulated target genes are shown as arrows and coloured as follows: grey, regulatory gene; yellow, gene involved in iron uptake and iron storage; orange, gene involved in central metabolism; red, gene contributing to the oxidative stress response. The highly conserved coregulation of the hmuO gene by DtxR and the response regulators HrrA and ChrA in corynebacteria is indicated [23]. The 19-bp consensus sequence of the DtxR-binding site of C. pseudotuberculosis FRC41 is shown as DNA sequence logo. The 16 predicted DNA-binding sites of DtxR were used as input data for the WebLogo tool [115].