Figure 6

Regulatory interactions involved in the control of potential virulence factors of C. pseudotuberculosis FRC41. Transcription regulators controlling the expression of candidate virulence factors are shown. The regulatory interactions were deduced from a genome-scale network transfer approach [114] and the combined use of a hidden Markov model and a position weight matrix [74]. The assignment of the transcription regulators into the regulatory protein families of C. pseudotuberculosis FRC41 is indicated. Predicted DNA-binding sites of RamA and RamB are shown as white boxes and predicted DNA-binding sites of GlxR as black boxes. Regulated target genes are shown as arrows and coloured as follows: grey, regulatory gene; yellow, gene involved in iron storage; orange, gene of central metabolism or fatty acid/mycolic acid biosynthesis; violet, gene involved in the resuscitation process; light green, gene involved in the assembly of adhesive pilus; dark green, other potential virulence factor gene. The 16-bp consensus sequence of the GlxR-binding site of C. pseudotuberculosis FRC41 is shown as DNA sequence logo. The 17 predicted DNA-binding sites of GlxR were used as input data for the WebLogo tool [115].