Prediction of planarian transmembrane proteins and functional annotations. A) Venn-diagram showing the overlap between predictions of transmembrane proteins generated by the Phobius, TMHMM2.0 and SOSUI programs for a set of 56,362 protein sequences translated from planarian ESTs. Only proteins predicted to contain one or more transmembrane domains by at least two programs (colored orange, 8,597 proteins, of which 4,663 are non-redundant) were considered for further analysis. B) Top ten PFAM domains and C) gene ontologies (biological process) for the 4,663 non-redundant transmembrane-proteins predicted. The figures indicate the number of proteins contained in a given annotation group.