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Figure 1 | BMC Genomics

Figure 1

From: Normalization with genes encoding ribosomal proteins but not GAPDH provides an accurate quantification of gene expressions in neuronal differentiation of PC12 cells

Figure 1

Neuronal differentiation of PC12 cells. A. Quantification of the percentage of PC12 cells bearing neurite of at least one cell body length, after 72 h of treatment with NGF (50 ng/ml), GDNF (50 ng/ml), Forskolin (10 μM), KCl (5 mM) and ROCK inhibitor Y27632 (25 μM). GDNF treatment was applied to PC12 cells stably expressing GDNF Family Receptor alpha 1a (GFRa1a) and co-receptor RET (either RET9 or RET51 isoforms), which were not endogenously expressed at detectable level in PC12 cells. All other stimulations were applied to wild type PC12 cells. B. Representative images of control and treated PC12 cells immuno-stained with anti-Neurofilament 200 antibody.

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BMC Genomics

ISSN: 1471-2164

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