Figure 6

Analysis of the p48-like sequence of M. agalactiae 5632 suggests a mechanism for phase variation. Schematic represents the p48-like genomic region (A). CDSs are represented by large arrows with MAGa1620 corresponding to p48-like gene filled in blue. Translation of the DNA region flanking the polyG track is given in the three frames (B). The polyG tract suspected to vary in length (G10 +/-1) is underlined by a bold red bar. The putative beginning of a P48-like lipoprotein with an entire signal peptide sequence is shaded in red while the current annotated MAGa1620 open reading frame is in blue. Global amino-acid alignment results obtained with Needle (program available at http://www.ebi.ac.uk/Tools/emboss/align/) between the P48-like of M. agalactiae 5632 and the P68 lipoprotein of M. bovis PG45 for which a similar polyG tract was previously described [48], are of 89.3% (identity) and 92.1% (similarity).