Comparative genomic and proteomic analyses of two Mycoplasma agalactiae strains: clues to the macro- and micro-events that are shaping mycoplasma diversity

Table 1 General properties of M. agalactiae PG2 and 5632 strains

	PG2	5632
Date of isolation	1952	<1991
Country	Spain	Spain
Source	nk	articulation
Host	caprine	caprine
Genome size (bp)	877,438	1,006,702
G+C (%)	29.70	29.62
Gene density (%)	88.5	88.7
Total number of CDS	752	826
HP (Hypothetical Protein)	138	148
CHP (Conserved HP)	186	150
CDS with predicted function	404	505
Pseudogenes	45	23
rRNAs sets	2	2
tRNAs	34	34
GenBank accession number	CU179680	FP671138
ICE number	(1 vestigial)	3 (+2 vestigial)
Transposase number	1^a	15
	(+2 pseudogenes)	(+2 pseudogenes)
Genomic DNA digested by:
Dpn I or Alw I (sens. to Dam methylation)	Yes	No
Dpn II (Dam resistant)	Yes	Yes
Relative colony size^b	100%	180%

Data were extracted from the MolliGen database http://cbi.labri.fr/outils/molligen/.
^a One CDS, MAG3410, was annotated as transposase and was detected by proteomics analysis in this study but no inverted repeat sequences could be found.
^bRelative colony sizes as defined on agar medium with PG2 as reference. Repeatedly colonies of 5632 were found to be approximately 1.8 times larger than those of PG2.
nk, not known

ISSN: 1471-2164