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Table 2 List of genes involved in the (oxidative) stress response of sessile B. cenocepacia J2315 cells exposed to H2O2 or NaOCl.

From: Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite

  

Fold change in expression in treated biofilms vs untreated biofilms

Gene number

Annotation

Microarray: H2O2(30 min)

qPCR: H2O2(15 min)

qPCR: H2O2(30 min)

qPCR: H2O2(60 min)

Microarray: NaOCl (5 min)

Genes involved in the oxidative stress response

BCAS0085

Organic hydroperoxide resistance protein

49.3

1438.4

1307.0

560.7

20.3

BCAM1217

Alkyl hydroperoxide reductase C subunit (ahpC)

41.3

68.7

46.7

20.4

15.0

BCAL0771

Non-heme chloroperoxidase

37.7

27.0

65.8

97.5

NS

BCAM0896

Organic hydroperoxide resistance protein

36.5

27.6

23.2

15.1

37.2

BCAL1766

OsmC-like protein (Ohr protein, see text)

18.0

28.2

43.8

36.9

75.2

BCAM1216

Alkyl hydroperoxide reductase F subunit (ahpF)

15.5

182.5

122.5

49.5

2.1

BCAM0931

Catalase precursor

5.7

98.8

32.3

6.1

NS

BCAL1106

Cytochrome b561 family protein

4.1

1.7

12.7

42.5

8.3

BCAL2780

Putative thioredoxin protein

2.9

1.9

3.1

3.3

NS

BCAL3035

Thioredoxin reductase (trxB)

2.8

-

-

-

NS

BCAM0930

Putative ankyrin-like protein (ankB)

2.7

-

-

-

NS

BCAL3477

Putative catalase

2.7

1.7

6.5

23.1

NS

BCAL2014

Carboxymuconolactone decarboxylase family

2.7

2.3

2.1

1.7

3.3

BCAL1761

MarR family regulatory protein (ohrR)

2.4

-

-

-

NS

BCAL2013

AhpC/TSA family protein

2.1

-

-

-

NS

BCAL1900

Thioredoxin A (trxA)

2.0

-

-

-

2.1

BCAL3299

Catalase (katB)

1.8

1.4

1.7

NS

NS

BCAM2107

Catalase (katA)

NS

NS

NS

NS

NS

BCAL3214

Carboxymuconolactone decarboxylase family

NS

-

-

-

35.1

Genes involved in protection and repair of DNA

BCAL0953

Putative recombinase A (recA)

2.6

NS

1.8

NS

1.9

BCAL3297

DNA-binding protein (dps)

NS

-

-

-

NS

Genes involved in the repair of iron-sulfur clusters and iron-sulfur cluster containing proteins

BCAM0961

Aconitase A (acnA)

NS

-

-

-

NS

BCAM1833

Aconitase B (acnB)

5.1

2.7

3.5

3.1

2.1

BCAL2192

Conserved hypothetical protein (iscX)

2.4

-

-

-

2.4

BCAL2193

Ferredoxin (fdx)

2.5

-

-

-

2.5

BCAL2194

Chaperone protein HscA homologue (hscA)

2.4

-

-

-

2.4

BCAL2195

Chaperone protein HscB homologue (hscB)

2.3

-

-

-

2.3

BCAL2196

Iron-sulfur cluster assembly protein (iscA)

1.6

-

-

-

1.6

BCAL2197

Iron-sulfur cluster scaffold protein (iscU)

3.4

-

-

-

3.4

BCAL2198

Cysteine desulfurase (iscS)

2.9

-

-

-

2.9

BCAL2199

Transcriptional regulator (iscR)

2.3

-

-

-

2.3

Sigma factors

BCAL0787

RNA polymerase σ32 factor

4.7

2.3

2.7

2.5

2.6

BCAL1369

Putative RNA polymerase σ70 factor (fecI)

2.2

-

-

-

NS

BCAL1688

Putative RNA polymerase σ70 factor (orbS)

6.4

5.7

5.9

12.9

NS

BCAL3478

Putative RNA polymerase σ factor

3.4

NS

5.5

16.6

NS

  1. The results represent the fold change in gene expression in the treated biofilms compared to the gene expression in the untreated biofilms. -: no qPCR experiments were performed. NS: no significant change in expression was observed between the treated and the untreated biofilms (p > 0.05).