Table 2 True positive rate at mass tolerance of 0.04 Da.
Type of methylation | True positive rate (%) | No. of methylation sites correctly matched | No. of methylation sites tested | No. of peptides tested |
|---|---|---|---|---|
a) Non-redundant known mono- and di-methylation | ||||
Monomethyl-K | 100 | 27 | 466 | 1,201 |
Dimethyl-K | 98 | 89 | 280 | 699 |
Monomethyl-R | N.D.a | N.D.a | 3 | 6 |
Dimeth-R | 76 | 28 | 137 | 495 |
b) Artificial mono- and di-methylation | ||||
Monomethyl-K | 78 | 217 | 11,377 | 30,782 |
Dimethyl-K | 89 | 3,673 | 11,377 | 30,691 |
Monomethyl-R | 90 | 453 | 6,941 | 18,728 |
Dimethyl-R | 90 | 2,140 | 6,902 | 18,594 |