Table 1 Variability and reproducibility after applying two common probe filters (detection score >0.95 and SD > 0.5) for each RNA and cell isolation method.
From: Variability of gene expression profiles in human blood and lymphoblastoid cell lines
RNA and cell isolation method | No. of probes with SD > 0.5 | No. of probes with detection score >0.95 | Spearman correlation range across replicates* Mean(range) | Spearman correlation range across random individuals Mean(range) |
|---|---|---|---|---|
PAX | 34,012 | 8,783 | 0.96 (0.96-0.98) | 0.93 (0.90-0.95) |
PBMC | 27,987 | 11,834 | 0.98 (0.90-0.99) | 0.96 (0.93-0.97) |
LCL | 24,311 | 11,865 | 0.99 (0.96-1.0) | 0.96 (0.96-0.97) |
Bcell CD19 | 24,229 | 12,122 | 0.99 (0.98-0.99) | 0.97 (0.93-0.96) |
Bcell CD20 | 30,342 | 10,672 | 0.96 (0.93-0.98) | 0.95 (0.96-0.97) |