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Table 1 Variability and reproducibility after applying two common probe filters (detection score >0.95 and SD > 0.5) for each RNA and cell isolation method.

From: Variability of gene expression profiles in human blood and lymphoblastoid cell lines

RNA and cell isolation method No. of probes with SD > 0.5 No. of probes with detection score >0.95 Spearman correlation range across replicates* Mean(range) Spearman correlation range across random individuals Mean(range)
PAX 34,012 8,783 0.96 (0.96-0.98) 0.93 (0.90-0.95)
PBMC 27,987 11,834 0.98 (0.90-0.99) 0.96 (0.93-0.97)
LCL 24,311 11,865 0.99 (0.96-1.0) 0.96 (0.96-0.97)
Bcell CD19 24,229 12,122 0.99 (0.98-0.99) 0.97 (0.93-0.96)
Bcell CD20 30,342 10,672 0.96 (0.93-0.98) 0.95 (0.96-0.97)
  1. * Spearman correlations are calculated between two visits for each matched or random individual for each method. Spearman correlations are based on detected probes.